中国兽医科学2012，Vol.42Issue(2)：182-185,4.
犊牛血清对高致病性猪繁殖与呼吸综合征病毒增殖及其活疫苗的影响
Influence of FCS on the replication of HP-PRRSV and the living vaccine against the virus
摘要
Abstract
To evaluate the effect of different content of FCS on the quality and amount of JXA1-R strain of HP-PRRSV,and the immunity effect of the vaccine produced from the strain,four samples with different FCS contents were used to reproduce the JXA1-R strain of HP-PRRSV,with the cytotoxic effect and antibody tilter detected,fever of the immuned pigs monitored,and the specific antibody level of PRRSV in serum detected with ELISA method.In result,the contents of virus produced had no major changes when the serum contents ranged from 15 to 30mL/L,with only slightly increase with the serum contents. And there were no significant changes in the virus contents of vaccines. Swine vaccinated with vaccine produced in higher content serum showed transitory high temperature fluctuations in two to four days,then revived to normal. And it was also found that the appearance of antibody induced by the vaccine produced in higher content serum was three to five days earlier. In conclusion,the research provides an important reference for the culture of HP-PRRSV and will improve the vaccine process.关键词
猪繁殖与呼吸综合征病毒/血清/疫苗Key words
porcine reproductive and respiratory syndrome virus(PRRSV)/serum/vaccine分类
农业科技引用本文复制引用
任培森,吕文静,丁壮..犊牛血清对高致病性猪繁殖与呼吸综合征病毒增殖及其活疫苗的影响[J].中国兽医科学,2012,42(2):182-185,4.基金项目
广东省科技攻关重大项目 （）
国家农业科技成果转化资金项目 （）
