中国兽医科学2012，Vol.42Issue(2)：186-191,6.
热胁迫下奶牛肝脾HSP70的表达特征及脾淋巴细胞凋亡
Expression profiles of HSP70 in liver and spleen of cows as well as spleen lymphocyte apoptosis under heat stress
摘要
Abstract
To investigate the effects of heat stress on histomorphology and function of cows,the histology of liver and spleen of initial stage of weaning calf under experimental heat stress was observed,the expression profiles of HSP70 in liver and spleen were analyzed,and the spleen lymphocyte apoptosis was also assayed.The result showed that the structure of liver and spleen in each group revealed no difference at normal temperature condition.Compared with 25℃ condition,cows had the larger bounds of microfold,the better structural and functional of red-eared slide and white-eared slide,the more compact lymphocyte arrangement of microfold in spleen,which reflected the difference in tissue structure and caused the difference in heat resistance.The different heat stress could damage liver and spleen at different degrees in all groups,and the ultrastructure changed with the heat stress times. The contents of HSP70 showed a curve change with the heat stress times and had a certainty relation with histiocyte damages,which inferred that the contents of HSP70 could be as one of indicators to determinate histiocyte damages.The damages degree of organs and tissues in cows with heat stress was gradually aggravated,part of lymphocyte were showed from apoptosis to putrescence as the hyperthermia stress increase,the damages of structures and cells in liver and spleen aggravated.关键词
热胁迫/奶牛/表达特征/细胞凋亡Key words
heat stress/cow/expression profile/apoptotic cell分类
农业科技引用本文复制引用
刘庆华,甘乾福,梁学武..热胁迫下奶牛肝脾HSP70的表达特征及脾淋巴细胞凋亡[J].中国兽医科学,2012,42(2):186-191,6.基金项目
科技部科技支撑计划项目 （）
福建省科技厅农业重点项目 （）
