中国兽医科学2012，Vol.42Issue(3)：221-226,6.
狂犬病病毒aG株G基因的克隆与生物信息学分析
Cloning and bioinformatics analysis of G gene of rabies virus aG strain
摘要
Abstract
In order to monitor the evolutions of rabies virus aG strain,and to analyze the genetic vari-ation and function of G gene of aG strain,the total RNA was extracted from the rabies virus aG strain cultured in BHK-21.The G gene was amplified by RT-PCR and directly cloned into pMD18-T simple vector.The plasmid was transformed into Escherichia coli JM109,and then identified by PCR amplification and sequencing.Then the function of G gene was predicted by bioinformatics software and the phylogenetic tree of G gene was constructed by MEGA 5 software.The results indicated the complete G gene was 1 575 bp in length,encoding 524 aa.Bioinformatics analysis showed that molecular weight of G protein was 58 377.22 u,and the theoretical pI,estimated half-life,instability index and fatty index of G protein was 7.080,30 hours（mammalian reticulocytes in vitro）,42.99 and 88.45,respectively.Grand average of hydropathicity of G protein was-0.106.The maximum and the minimum hydropathicity were 3.489 and-2.600 respectively.The signal peptide of the protein was 1-19 amino acid residues.Motif searching showed that G protein might have 4 N-glycosylation sites,2 cAMP and cGMP protein kinase phosphorylation sites,13 protein kinase C phosphorylation sites,12 casein kinaseⅡ phosphorylation sites,1 tyrosine protein kinase phosphorylation site,9 N myristoylation sites,and 1 amidation site.Analysis of phylogenetic tree indicated that the aG strain shared the closest evolutionary distance with rabies virus DRV strain.关键词
狂犬病病毒/G基因/生物信息学分析Key words
rabies virus/G gene/bioinformatics analysis分类
农业科技引用本文复制引用
焦文强,关洪鑫,殷相平,柳纪省..狂犬病病毒aG株G基因的克隆与生物信息学分析[J].中国兽医科学,2012,42(3):221-226,6.基金项目
国家高技术研究发展计划（863）项目 （）
