中国兽医科学2012，Vol.42Issue(3)：264-267,4.
水貂肠炎病毒疫苗株和野毒株PCR-RFLP鉴别方法的建立
Establishment of PCR-RFLP for differentiation of mink enteritis virus vaccine strain from wild strain
摘要
Abstract
To establish a PCR-RFLP method for the differentiation of mink enteritis virus wild strain from vaccine strain,a pair of primers was designed according to the nucleotide sequence of mink enteritis virus available in the GenBank.The NS1 genes of mink enteritis viruses including wild and vaccine MEVB strain,other wild canine parvovirus and feline panleukopenia virus strains were amplified and analyzed.The result showed the substitution of T to C in 1401 site,and an additional restriction site of ClaⅠwas found in the vaccine MEVB strain,while it did not exist in other reference strains.The PCR product of NS1 gene of vaccine MEVB strain can be digested by ClaⅠ 2 fragments of 1 404 bp and 609 bp,respectively;however,the wild strains can not digested.Therefore,the method could be used to differentiate the mink enteritis virus wild strain from the vaccine strain.关键词
水貂肠炎病毒/聚合酶链反应-限制性片段长度多态性检测法/疫苗株/野毒株Key words
mink enteritis virus/PCR-RFLP/vaccine strain/wild strain分类
农业科技引用本文复制引用
王建科,程世鹏,杨莘,易立,许红丽,程悦宁,师新川,武华,闫喜军..水貂肠炎病毒疫苗株和野毒株PCR-RFLP鉴别方法的建立[J].中国兽医科学,2012,42(3):264-267,4.基金项目
农业科技成果转化资金项目 （）
吉林省重大科技成果转化项目 （）
