中国兽医科学2012，Vol.42Issue(3)：275-279,5.
旋毛虫HSP70基因的原核表达及其免疫原性
Prokaryotic expression of HSP70 gene of Trichinella spiralis and immunogenicity analysis of the expression product
摘要
Abstract
HSP70 gene of Trichinella spiralis was amplified by RT-PCR and then cloned into pEASY-Blunt Simple vector.The recombinant was digested and linked with pET-28a（＋） vector.The recombinant plasmid was confirmed by PCR and DNA sequencing.The positive plasmid was transformed into competent Escherichia coli Transetta（DE3） and induced by IPTG for expression.The recombinant Trichinella HSP70 was purified to prepare polyclonal antibody.Trichinella HSP70 was analysed by Western-blot and stained by immunohistochemistry.The recombinant protein was confirmed to be about 70 ku in size.The positive signals were localized in the Trichinella by immunohistochemistry.The results showed that the recombinant expression plasmid pET28a-HSP70 was successfully constructed and laid the foundation for further studies on the function of HSP70.关键词
旋毛虫/热休克蛋白70/原核表达Key words
Trichinella/HSP70/prokaryotic expression分类
农业科技引用本文复制引用
徐佳,禹洋,唐颖,俞昭旸,李晓云,宋铭忻..旋毛虫HSP70基因的原核表达及其免疫原性[J].中国兽医科学,2012,42(3):275-279,5.基金项目
“十一五”国家科技支撑计划重大项目 （）
国家自然科学基金资助项目 （）
家畜疫病病原生物学国家重点实验室开放课题 （）
