中国兽医科学2012，Vol.42Issue(3)：290-293,4.
牦牛源多杀性巴氏杆菌外膜蛋白H重组亚单位疫苗对小鼠的免疫保护效果
Immune efficacy of recombinant subunit vaccine with outer membrane protein H of Pasteurella multocidafrom yak in mice
摘要
Abstract
To evaluate the immunogenicity of outer member protein H（OmpH） of yak Pasteurella multocida,vaccines were prepared with the purified recombinant OmpH（rOmpH） of the P.multocida.Mice were hypodermically immunized with the rOmpH vaccine and inactivated whole bacteria vaccine,with PBS as control.Sera were collected weekly from the mice and antibody responses were detected by indirect ELISA.The immunized mice were challenged with 2 LD50 P.multocida on day 9 after the third immunization to observe the protective efficacy.The results showed that 20% of the inactivated whole bacteria vaccine groups died after 30 h post-challenge,and 100% of the recombinant vaccine groups died after 30 h,and 100% of the PBS groups died after 24 h.The results showed that inactivated whole bacteria vaccine was shown to induce higher immune protection,but the rOmpH vaccine has lower immune protection.关键词
牦牛/多杀性巴氏杆菌/OmpH/免疫保护力Key words
yak/Pasteurella multocida/OmpH/protective efficacy分类
农业科技引用本文复制引用
田亮,康立超,赵文娟,薄新文,马勋..牦牛源多杀性巴氏杆菌外膜蛋白H重组亚单位疫苗对小鼠的免疫保护效果[J].中国兽医科学,2012,42(3):290-293,4.基金项目
新疆农垦科学院科技引导计划项目 （）
新疆农垦科学院青年基金项目 （）
