中国兽医科学2012，Vol.42Issue(3)：309-313,5.
绵羊胎盘滋养层细胞的体外培养与鉴定
Culture and identification of sheep trophoblast cells in vitro
摘要
Abstract
To establish a stable and convenient method for preparation of highly purified trophoblast cells from sheep placenta and to provide the cellular basis for the study of cell transfection and RNA interference test,the sheep trophoblast cells were cultured by explant culture technique,and purified by digestion differential method.Phase-contrast microscope was used to observe the morphological characteristics of trophoblast cells,and general HE staining,immunohistochemical staining,electron microscopy and RT-PCR technology were used to identify the cell source.Inverted phase contrast microscope observation showed that the cells were the epithelial-like cell morphology,paving-like growth.Keratin staining showed that the purity of the cultured cells exceeded 90%,vimentin staining was negative.The unique structure of trophoblast cells could be observed by transmission electron microscopy.The cell viability evaluated by trypan blue exclusion test was greater than 95%.Conventional RT-PCR technique can detect trophoblast cells secreted interferon specific protein-1 gene.The results showed that this primary culture method can provide highly purified sheep trophoblast cells with good cellular viability.The present study provides an experimental basis for our laboratory in vitro studies.关键词
绵羊/滋养层细胞/细胞培养/鉴定Key words
sheep/trophoblast cell/cell culture/identification分类
生物科学引用本文复制引用
刘慧,刘淑英,刘丽珍,张宇飞..绵羊胎盘滋养层细胞的体外培养与鉴定[J].中国兽医科学,2012,42(3):309-313,5.基金项目
国家自然科学基金项目 （）
内蒙古科技创新引导基金项目 （）
