中国兽医科学2012，Vol.42Issue(4)：413-416,4.
鸡胚胎腺垂体卵泡刺激素细胞的发育及其变化
Ontogeny and the developing change of pituitary follicle-stimulating hormone cells of chicken embryos
摘要
Abstract
The present study is to determine ontogeny of chick embryonic pituitary follicle-stimulating hormone（FSH） cells and their change during developing embryos.The pituitary glands were collected on day 3.5 to 20.5 of incubation,respectively.The expression and distribution of pituitary FSH cells were then detected by the immunohistochemical method.The experimental results demonstrated that scattered and clarity immunopositive FSH cells were first detected in caudal lobe of pituitary gland on day 10.5,and then dramatically increased（P0.05）.FSH cells increased to a peak on day 16.5,and distributed caudal lobe,only scattered FSH cells distributed cephalic lobe of pituitary gland.On day 18.5 FSH cells decreased.In the early stage,the volume of FSH cells was smaller,there was less cytoplasmic and bigger nuclear,and light staining;in the mid-incubation and later,the volume become bigger,the cells became intensive staining.These results suggested that the ontogeny of FSH cells is in the middle stage and that the proliferation and differentiation of FSH cells occur during middle and late stage.关键词
鸡胚胎/腺垂体/卵泡刺激素细胞/免疫组织化学Key words
chick embryo/pituitary gland/follicle-stimulating hormone cells/immunohistochemical分类
农业科技引用本文复制引用
何玉琴,刘英,袁学军,刘靖闻,陈慧,吴瑜瑜..鸡胚胎腺垂体卵泡刺激素细胞的发育及其变化[J].中国兽医科学,2012,42(4):413-416,4.基金项目
甘肃省高等学校研究生导师科研项目 （）
