中国兽医科学2012，Vol.42Issue(5)：441-447,7.
RGD基序邻近氨基酸突变对口蹄疫病毒O/JC/2010株毒力的影响
Role of the amino acids adjacent to RGD motif in the virulence of foot-and-mouth disease virus strain O/JC/2010
摘要
Abstract
In order to study the role of neighboring amino acids of RGD motif in the virulence of footand-mouth disease virus（FMDV） ,the VPI coding region of O/JC/2010 strain was used to substituted for counterpart of genetically engineered virus O/HN/93. The chimeric FMDV strain（named pBlue-HJ） was got by reverse genetic technology. Besides, four chimeric FMDV strains pBlue-HJ （142）, pBlue-HJ （142 ＋ 152） ,pBlue-HJ（152＋ 153）, pBlue-HJ （142 ＋ 152 ＋ 153） were constructed through a serious of mutations （P142T,A152D and Q153P） in VP1. Mutations P142T and A152D were beneficial to virulence increasing in the BHK-21 cell and sucking mouse, nevertheless, Q153P went against virulence increasing. The results showed that the nonconservative amino acid sites adjacent to RGD motif could affect the virulence of FMDV. The study lays a foundation for further study of molecular pathogenesis of FMDV.关键词
口蹄疫病毒/G-H环/RGD基序/毒力Key words
foot-and-mouth disease virus/G-H loop/RGD motif/virulence分类
农业科技引用本文复制引用
况文东,白兴文,李平花,祁国财,包慧芳,芦曾军,孙普,谢宝霞,郝晓芳,刘在新..RGD基序邻近氨基酸突变对口蹄疫病毒O/JC/2010株毒力的影响[J].中国兽医科学,2012,42(5):441-447,7.基金项目
公益性行业（农业）科研专项 （）
