中国兽医科学2012，Vol.42Issue(5)：483-487,5.
小反刍兽疫病毒N蛋白主要抗原区域的原核表达及间接ELISA检测方法的建立
Prokaryotic expression of main antigen region of N protein of peste des petits ruminants virus and the development of indirect ELISA
摘要
Abstract
To develop a method for specific detection of serum antibody against peste des petits ruminants virus（PPRV）,a pair of primers was designed to amplify the fourth region of PPRV N protein which was specific to PPRV.The target segment was cloned into the pET-32a（＋） expression vector to get recombinant protein.Western-blot analysis showed that the recombinant protein had immunoreactivity,and could be used as coating antigen to develop an indirect ELISA to detect the serum antibody against PPRV.Reaction conditions in the indirect ELISA such as antigen coating concentration,the dilution of sera,reaction time of sera were optimized.The established indirect ELISA was then used to detect serum antibody against PPRV from rinderpest virus,which indicated that the method was sensitive and specific.Compared with standard competitive ELISA,the coincidence rate between the two methods was 98.4%.An indirect ELISA to detect IgG against PPRV in sera specifically was established successfully.关键词
小反刍兽疫/N蛋白/间接酶联免疫吸附试验Key words
peste des petits ruminants(PPR)/N protein/indirect ELISA分类
农业科技引用本文复制引用
邱文英,李刚,田康乐,黄华欣..小反刍兽疫病毒N蛋白主要抗原区域的原核表达及间接ELISA检测方法的建立[J].中国兽医科学,2012,42(5):483-487,5.基金项目
国家高技术研究发展计划（863）项目 （）
FAO/IAEA项目（14515 （）
14133） （）
国家公益性行业科研专项 （）
“十一五”国家科技支撑计划项目 （）
