中国兽医科学2012，Vol.42Issue(5)：523-528,6.
内含肽和麦芽糖结合蛋白介导具有抗病毒活性牛α干扰素的表达
Expression of recombinant bovine interferon-a with antiviral activity by using intein induced expression system
摘要
Abstract
To achieve high-level expression of soluble recombinant bovine interferon-a（BoIFN-a） in Escherichia coli ,the Ssp DnaB intein（SDI） coding sequence was merged into the upstream of the BoIFN-a cDNA,and then cloned into the expression vector pMAL-c2X to construct the intein expression plasmid pMAL-SDI-BoIFN-a. The recombinant expression plasmids were transformed into E. coli Rosetta and induced by IPTG. The result showed that the fusion protein was expressed in the form of solubility and more pure proteins can be obtained by Amylose Resin column purification. The purified protein shaved the antiviral activity in MDBK-BVDV（bovine viral diarrhea virus） system.关键词
牛α干扰素/内含肽/麦芽糖结合蛋白/可溶性表达/抗病毒活性Key words
bovine IFN-a/intein/maltose-binding protein/soluble expression/antiviral activity分类
农业科技引用本文复制引用
崔子寅,王艺萌,唐博,孙旭燕,高明春,王君伟..内含肽和麦芽糖结合蛋白介导具有抗病毒活性牛α干扰素的表达[J].中国兽医科学,2012,42(5):523-528,6.基金项目
国家奶牛产业技术体系建设专项资金项目 （）
动物生物制品共性技术应用研究 （）
