中国畜牧杂志2011，Vol.47Issue(21)：21-25,5.
BMP-4mRNA在兔卵巢中的定位及其在卵母细胞体外成熟培养中的表达研究
In Situ Hybrization Localization of Bone Morphogenetic Protein 4 mRNA in the Rabbit Ovary and Its Expression During in vitro Maturation of Oocytes
摘要
Abstract
BMP-4 protein has the function of regulation of ovarian folliculogenesis. In order to investigate the expression pattern of BMP-4, in the rabbit ovary and reveal its functions in some processes, such as the maturation of oocytes ,de-velopment and luteinization of follicles, and etc. We examined the localization of BMP-4 mRNA in the rabbit ovary by in situ hybrization method. The results showed that BMP-4 mRNA was expressed in oocytes of non-atretic follicle weakly, and was not expressed in their granulosa cells, theca cells and Cumulus cells.But BMP-4 mRNA was strongly expressed in oocytes of atretic -tertiary follicles. During in vitro maturation (IVM), the expression of BMP-4 mRNA was measured in rabbit oocytes by fluorescent quantitative RT-PCR. Gene BMP-4 was expressed at a low level,and decreased to the lowest level at 16 h of IVM. It was Conjected that BMP-4 protein may be one of an inization inhibitor and play an important role having antagonistic effect with BMP-15 protein in cumulus expansion of rabbit. In addition,High activity BMP-4 protein possibility take part in the mechanism of follicular atresia of rabbit by high autocrine of oocytes.关键词
骨形态发生蛋白4/卵母细胞/原位杂交/荧光定量/兔Key words
bone morphogenetic protein 4/ oocytes/ in situ hybrization/ fluorescent quantitative RT-PCR/rabbit分类
农业科技引用本文复制引用
李霖,沈曼曼,曹卉,尹萍,季金强,丁家桐..BMP-4mRNA在兔卵巢中的定位及其在卵母细胞体外成熟培养中的表达研究[J].中国畜牧杂志,2011,47(21):21-25,5.基金项目
江苏省自然科学基金(BK200505) （BK200505）
