中国畜牧杂志2012，Vol.48Issue(5)：1-6,6.
鸡GPC1基因外显子8的SNPs与部分免疫性状的相关性分析
Association between Single Nucleotide Polymorphisms of Exon 8 of GPC1 Gene and Immune Traits of Chicken
摘要
Abstract
The PCR-SSCP technology and DNA sequencing was conducted to detect single nucleotide polymorphisms(SNPs) of exon 8 in glypican-1 (GPC1) gene in twelve breeds. Five immune traits and their associations with SNPs were also detected at 56 days of age in Wenchang, Rugao and Anka chickens. 1 non-synonymous SNP, g.155 C>A, were found, resulting in amino acid sequence altered from Threonine to Arginine. In all 12 breeds, the average frequencies of AA, AB and BB genotypes were 0.259, 0.545, and 0.211, respectively, and the average value of observed heterozygosis and Shannon's index were 0.4658 and 0.6710. Except Taihe chicken and Dagu chicken, the other populations were all in Hardy-Weinberg equilibrium at this locus (P>0.05). The dendrograms divided the 12 chicken populations into 3 clusters, which reflected the resistance difference between these 12 breeds. IL-1 concentrations and H / L values of AB genotype individuals was significantly differ from that of BB genotype individuals, rather than that of AA genotype individuals in Rugao and Anka chickens. There was no significance in SRBC antibody titer, ND antibody and Al antibody titer between three genotypes. In general, the disease resistance of Rugao and Wenchang were better than that of Anka chicken.关键词
GPC1基因/单核苷酸多态性/免疫性状/鸡Key words
GPC1 gene/SNP/immune traits/chicken分类
农业科技引用本文复制引用
盛中伟,常国斌,栾德琴,周伟,胡国顺,刘艳,李芹,陈国宏..鸡GPC1基因外显子8的SNPs与部分免疫性状的相关性分析[J].中国畜牧杂志,2012,48(5):1-6,6.基金项目
江苏省自然科学基金(BK2009190) （BK2009190）
江苏省科技支撑计划(BE2010375) （BE2010375）
