中国畜牧杂志2012，Vol.48Issue(7)：23-25,3.
鸡胚发育过程中胚胎液中几种游离氨基酸的动态变化
Developmental Changes of Several Free Amino Acids in Fetal Fluids of Chickens
摘要
Abstract
Seventy-two three-Yellow chicken breeding eggs were used to investigate the developmental change of free aspatic acid, glutamic acid, arginine and leucine concentrations in allantoic and amniotic fluids during breeding egg incubation. The allantoic and amniotic fluids were obtained at 7, 8, 11, 13, 14 and 18 day, and analyzed by High Performance Liquid Chromatography. The results showed that except on day 8, arginine concentrations were higher than the other three free amino acids in allantoic fluid. The arginine concentration was the lowest on day 13 and the highest on day 18 (P<0.05). The aspatic acid, glutamic acid and leucine concentrations reached the peak value on day 8 (P<0.05). Arginine was the most abundant free amino acids in amniotic fluid and reached the highest value on day 13 (P<0.05). Compared to day 7, the aspatic acid concentration in amniotic fluid was significantly decreased on day 18 (P<0.05), however, the glutamic acid and leucine concentrations were significantly increased (P<0.05). These results suggest that arginine is the most abundant compared to others three amino acids in allantoic and amniotic fluids, which may be related to nutrition and metabolism of chicken embryo development.关键词
胚胎液/氨基酸/高效液相色谱/三黄鸡Key words
fetal fluids/ arginine/ high performance liquid chromatography/ three-yellow chicken分类
农业科技引用本文复制引用
古殿超,朱惠玲,刘玉兰,姚文旭,夏强,周世超..鸡胚发育过程中胚胎液中几种游离氨基酸的动态变化[J].中国畜牧杂志,2012,48(7):23-25,3.基金项目
教育部科学技术研究重点项目(209082) （209082）
武汉工业学院研究生创新基金项目(2010cx006) （2010cx006）
武汉工业学院大学生创新项目(Cx201019) （Cx201019）
