中国药房2012，Vol.23Issue(11)：1010-1012,3.DOI:10.6039/j.issn.1001-0408.2012.11.19
应用HPLC法探究虎杖泻心汤中蒽醌类成分的含量
Exploration of Content Determination of Anthraquinones Components in Huzhang Xiexin Decoction by HPLC
摘要
Abstract
OBJECTIVE: To explore the content of anthraquinones in Huzhang xiexin decoction after Polygonum cuspidatum in-stead of Radix Et Rhizoma Rhei is combined with Scutellaria baicalensis and Rhizoma Coptidis. METHODS: HPLC method was adopted. Welchrom-C18(250 mm×4.6 mm, 5 μm) column was used with mobile phase composed of methanol-1% phosphoric acid (85:15) at the flow rate of 1.0 mL·min-1. Detection wavelength was set at 440 nm and column temperature was 30℃. RESULTS: The linear range of 5 anthraquinones was 0.007 46～0.111 90 μg for rhein, 0.007 88～0.118 20 μg for aloe-emodin, 0.025 12~ 0.376 80 μg for emodin, 0.007 72～0.115 80 ug for chrysophanol and 0.004 06~0.060 90 μg for physcion (r>0.999 0). The con-tent of emodin in Huzhang xiexin decoction was higher, while the decoction rate of rhein was low. CONCLUSION: The study can provide reference for further study of P. Cuspidatum.关键词
高效液相色谱法/虎杖/泻心汤/蒽醌类成分/含量Key words
HPLC/ Polygonum cuspidatum/ Xiexin for further study of P. Cuspidatum. Decoction/ Anthraquinones/ Content分类
医药卫生引用本文复制引用
施翠英,邓放,任波..应用HPLC法探究虎杖泻心汤中蒽醌类成分的含量[J].中国药房,2012,23(11):1010-1012,3.基金项目
四川省中医药管理局资助项目(200621) （200621）
