中国药房2012，Vol.23Issue(12)：1136-1139,4.DOI:10.6039/j.issn.1001-0408.2012.12.30
HPLC法测定不同厂家清肺抑火片中5种蒽醌类衍生物的含量
Content Determination of 5 Anthraquinone Derivatives in Qingfei Yihuo Tablets from Different Factories by HPLC
摘要
Abstract
OBJECTIVE: To develop a method for the content determination of 5 anthraquinone derivatives (aloe-emodin, rhe-in, emodin, chrysophanol and physcion) in Qingfei yihuo tablets, and to compare the content difference of 5 anthraquinone compounds in Qingfei yihuo tablets from different factories. METHODS: The determination was performed on Agela Venusil XBP-C18 column with acetonitrile-0.1% phosphoric acid as mobile phase (gradient elution) at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 226 nm, and the injection volume was 5 μL; the temperature of column was set at 40 ℃. RESULTS: The linear ranges were 0.010~0.200 ug for aloe-emodin (r=0.999 4), 0.040-0.800 μg for rhein (r=0.999 3), 0.040~0.800 μg for emodin (r=0.999 2), 0.040~0.800 ug for chrysophanol (r=0.999 3), 0.020-0.400 ug for physcion (r=0.999 6). The average recoveries were 102.95% (RSD=2.13%), 106.47% (RSD=1.88%), 106.48% (RSD=1.41%), 101.47% (RSD=1.45%) and 100.31% (RSD=1.88%), respectively (n=9). CONCLUSIONS: The method developed is accurate, reliable and reproducible, and can be used for quality control of Qingfei yihuo tablets. The content of anthraquinone in Qingfei yihuo tablets from different factories was different, which may result from different raw materials and production process.关键词
清肺抑火片/高效液相色谱法/蒽醌/含量测定Key words
Qingfei yihuo tablets/ HPLC/ Anthraquinone/ Content determination分类
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付艳芬,王剑华,周浓,李文美..HPLC法测定不同厂家清肺抑火片中5种蒽醌类衍生物的含量[J].中国药房,2012,23(12):1136-1139,4.
