中国药房2012，Vol.23Issue(13)：1169-1171,3.DOI:10.6039/j.issn.1001-0408.2012.13.06
人脐带间充质干细胞对CCl4致大鼠肝硬化的保护作用研究
Protective Effect of Human Umbilical Cord Mesenchymal Stem Cell on Hepatocirrhosis Rats Induced by CCl4
摘要
Abstract
OBJECTIVE: To investigate the protective effect of human umbilical cord mesenchymal stem cells (HUC-MSCs) on hepatocirrhosis in rats and its mechanism. METHODS: Rats hepatocirrhosis model was induced by subcutaneous injection of Ccli. Model rats were given half dose of CC14 continuously after modeling. The experimental animals were randomly divided into model group, normal saline (NS) group and HUC-MSCs group (l×107 counts/mL) with 24 rats in each group. The latter 2 groups were given intravenous injection of drug or cell 1 mL via tail vein. After transplantation, respectively at the 1st, 3rd, 5th weekend rats were sacrificed for detecting pathological changes of liver tissue, the levels of ALT, AST, CHO, ALB, TGF-p\, HGF and EGF-R. Other 8 rats were involved in normal control group. RESULTS: There was similar condition in model group and NS group. Collagen fibre proliferated obviously in liver, and liver cell disarranged. In HUC-MSCs group, 5 weeks after transplantation the proliferation of collagen fibre in liver decreased and there were many normal liver cell; compared with normal control group, ALT, AST, TGF-Pi and HGF levels increased significantly in model group and NS group, and the contents of CHO and ALB decreased significantly (P<0.01). Compared with model group, above parameters of HUC-MSCs group improved significantly (P<0.05 or P< 0.01) ; ALT, ALB and TGF-β1 levels were positively correlated with time after transplantation. The positive expression of human EGF-R was found in liver tissue of rats in HUC-MSCs group at 1, 3, 5 weeks after transplantation. CONCLUSION: HUC-MSCs transplantation can promote the repair of liver function and repair liver lesions induced by hepatocirrhosis.关键词
脐带间充质干细胞/肝硬化/大鼠/保护作用Key words
Umbilical cord mesenchymal stem cell/Hepatocirrhosis/Rats/Protective effect分类
医药卫生引用本文复制引用
李晓飞,金旭鹏,郭莲怡..人脐带间充质干细胞对CCl4致大鼠肝硬化的保护作用研究[J].中国药房,2012,23(13):1169-1171,3.基金项目
2010年辽宁省教育厅科研项目(L2010270) （L2010270）
