中国药房2012，Vol.23Issue(13)：1182-1184,3.DOI:10.6039/j.issn.1001-0408.2012.13.11
重组人p53腺病毒注射液逆转人耐药胃癌MGC-803细胞耐药性的体外试验研究
Experimental Research on Drug Resistance Reversal of Human Gastric Cancer MGC-803 by Recombinant Human Ad-p53 Injection in Vitro
张林 1王季堃 2王莉3
作者信息
- 1. 辽宁医学院护理学院,辽宁锦州121001
- 2. 辽宁医学院附属第一医院肿瘤科,辽宁锦州 121001
- 3. 辽宁省锦州市中心医院,辽宁锦州 121001
- 折叠
摘要
Abstract
OBJECTIVE: To discuss the reversal effects and the possible mechanism of recombinant human Ad-p53 injection (rAd-p53) on human gastric cancer MGC-803. METHODS: Drug resistant gene expressions of MGC-803 cells were induced by paclitaxel from low to the high dose. After the application of different concentrations of rAd-p53 on MGC-803 for different time, MTT method was used to measure inhibition rate of cell proliferation to calculate IC50. Cell cycle phase distribution and apoptosis were determined by flow cytometry. Immunohistochemistry and Western blot assay were used to evaluate the level of MDRl-Pgp expression. RESULTS: MTT method showed that rAd-p53 could inhibit the proliferation of MGC-803 in time and dose dependant manner. The inhibiting concentration of 50% cell growth (IC50) at 24 h, 48 h and 72 h were 1 889.85, 998.44, 354.91MOI, respectively. Cell cycle was arrested at the G2/M phase, and apoptosis of cells could be efficiently induced by rAd-p53 at different concentrations. MDRl-Pgp protein expression were down-regulated during rAd-p53 induction. CONCLUSION: Drug resistance of MGC-803 is mainly related with the high expression of MDRl-Pgp; rAd-p53 significantly inhibits the proliferation of MGC-803 and induce cell apoptosis in dose and time dependent manner.关键词
重组人p53腺病毒注射液/人耐药胃癌MGC-803细胞/细胞凋亡/逆转/耐药性Key words
Recombinant human Ad-p53 injection/Human gastric cancer MGC-803/Cell apoptosis/Reversal/Drug resistance分类
医药卫生引用本文复制引用
张林,王季堃,王莉..重组人p53腺病毒注射液逆转人耐药胃癌MGC-803细胞耐药性的体外试验研究[J].中国药房,2012,23(13):1182-1184,3.
