中国药房2012，Vol.23Issue(13)：1185-1187,3.DOI:10.6039/j.issn.1001-0408.2012.13.12
叶酸抑制大剂量甲氨蝶呤细胞毒性的试验研究
Study on the Inhibition Effect of Folic Acid on the Cytotoxicity of High-dose Methotrexate
王欢 1蔡绍晖 1潘琛 2梁巧 1吴肖蒙 1张志敏 1王波栋 1吴晓松3
作者信息
- 1. 暨南大学药学院,广州 510632
- 2. 黑龙江省中医研究院,哈尔滨150036
- 3. 暨南大学附属第一医院,广州 510630
- 折叠
摘要
Abstract
OBJECTIVE: To study the inhibition effect of folic acid (FA) on cytotoxicity of high-dose methotrexate (MTX). METHODS: The amount of MTX uptake was determined by cell uptake experiment of Caco-2 cells with HPLC-MS/MS technology after Caco-2 cells incubated with 1 umol·L-1 MTX in 1, 10, 100 umol·L-1 of FA for 30 min. Control group without FA was established with the drug uptake of cell/medium (C/M) ratio as index. RESULTS: After 30 min incubation, C/M=5.13 (n=4) in control group without FA, while the C/M=4.91, 4.22 or 3.74 in 1, 10, 100 umol·L-1 of FA (n=4), respectively. Compared with the control group without FA, the proportion of MTX uptake decreased significantly (P><0.05) in the group with 100 μmol·L-1 FA. CONCLUSION: When the concentration of FA is up to 100 umol-L"1, it can inhibit the cytotoxicity of high-dose MTX (1 umol· L-1) and protect the cells to some extent.关键词
甲氨蝶呤/叶酸/Caco-2细胞/细胞毒性/高效液相联用双质谱Key words
Methotrexate/Folic acid/Caco-2 cells/Cytotoxicity/HPLC-MS/MS分类
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王欢,蔡绍晖,潘琛,梁巧,吴肖蒙,张志敏,王波栋,吴晓松..叶酸抑制大剂量甲氨蝶呤细胞毒性的试验研究[J].中国药房,2012,23(13):1185-1187,3.
