中国药房2012，Vol.23Issue(14)：1280-1283,4.DOI:10.6039/j.issn.1001-0408.2012.14.12
我院2008-2010年抗菌药物应用与细菌耐药性分析
Analysis of the Utilization of Antibacterial Drugs and Bacterial Resistance in Our Hospital from 2008 to 2010
奚彩萍 1承晓京 1任俊杰2
作者信息
- 1. 南京医科大学附属常州市妇幼保健院,江苏常州213003
- 2. 南京中医药大学,南京210046
- 折叠
摘要
Abstract
OBJECTIVE: To develop a method for simultaneous determination of 4 lysophosphatidic acids (LPA) in human saliva. METHODS: LC-MS method was adopted. The saliva samples were extracted by HCl-acidified 1-butanol using LPA 17:0 as internal standard. The separation was achieved on Nucleodur 100-5 C8 column with mobile phase composed of methanol-2.5 mmol·L-1 ammonium formate buffer(Ph adjusted to 3.0 using formic acid) (85:15) at a flow rate of 0.5 Ml-muT1. The mass transition of mlz 409.0→153.0, 437.0→153.0, 435.0→153.0, 457.0→153.0, and 423.0→-153.0 were used to detect LPA 16=0,18:0,18:1,20:4,17:0, respectively. RESULTS: The linear range of all LPAs was 5~2 000 μg · L-1. Mean extraction recoveries were in the range of 79.91%~90.01%. The mean method recoveries were in the range of 92.16%~106.37%. RSDs of intra-day and inter-day conwere lower than 15%. CONCLUSION: The method is sensitive, simple and rapid, and it is suitable for the determination of LPAs concentration in human saliva.关键词
抗菌药物/用药频度/细菌耐药Key words
Lysophosphatidic acids/LC-MS/Saliva分类
医药卫生引用本文复制引用
奚彩萍,承晓京,任俊杰..我院2008-2010年抗菌药物应用与细菌耐药性分析[J].中国药房,2012,23(14):1280-1283,4.
