中国药房2012，Vol.23Issue(15)：1373-1375,3.DOI:10.6039/j.issn.1001-0408.2012.15.11
红花中羟基红花黄色素A的分离纯化工艺
Separation and Purification Technology of Hydroxysafflor Yellow A from Carthamus tinctorius
摘要
Abstract
OBJECTIVE: To separate and purify hydroxysaffl yellow A (HSYA) from Carthamus tinctorius. METHODS: HSYA was separated from C. Tinctorius and purified by using ZTCII as clarifier. The type modet of macroporous resin, separation technology and purification technology were screened with adsorption quantity, adsorption rate and desorption rate as index. RESULTS: X-5 macroporous resin was adopted. The separation technology was as follows: absorptive flow rate of 1 mL-miiT1, the best absorptive pH value of 3, 2% of column volume, 50% ethanol as elution solvent. The purification technology of polyamide resin was as follows: diameter-height ratio of 1:14, 2% of column volume, flow rate of 1.5 mL-min"', ethyl acetate-methanol-3.6% hydrochloric acid (1:3 = 6) as elution solvent, 6 times. The yield of HSYA was 25.63% and the purity of it was 83.65%. CONCLUSION: The technology is cheap and high purity in product. It is effective method to prepare HSYA.关键词
红花/羟基红花黄色素A/分离/纯化Key words
Carthamus tinctorius/ Hydroxysafflor yellow A/ Separation/ Purification分类
医药卫生引用本文复制引用
唐红,董志,曹纬国,肖晓秋,刘媛,程玉洁,王乐乐..红花中羟基红花黄色素A的分离纯化工艺[J].中国药房,2012,23(15):1373-1375,3.基金项目
国家科技重大专项重大新药创制(2010ZX09401-306-1-1) （2010ZX09401-306-1-1）
重庆市科委重点项目(CSTC2009BA5086) （CSTC2009BA5086）
