HPLC法测定蒙药蓝盆花中木犀草素和芹菜素的含量OACSCDCSTPCD
Content Determination of Luteolin and Apigenin in Mongolian Medicine Scabiosa tschilliensis by HPLC
目的:建立测定蒙药蓝盆花中木犀草素和芹菜素含量的方法.方法:采用高效液相色谱法.色谱柱为Phenomenex ODS-C18(250 mm× 4.6 mm,5μm),流动相为甲醇-0.4％磷酸(59∶41),流速为1 mL· mn-1,柱温为30℃,检测波长为345nm.结果:木犀草素、芹菜素的进样量分别在0.143～0.857 μg(r=0.999 5)、0.143～0.855.μg(r=0.999 6)范围内与各自峰面积积分值呈良好线性…查看全部>>
OBJECTIVE: To establish the method for the content determination of luteolin and apigenin in Scabiosa tschilliensis. METHODS: HPLC method was adopted. Phenomenex ODS-Ci?(250 mmx4.6 mm,5 um)column was used with methanol-0.4% H3PO4(59:41) as mobile phase at the flow rate of 1 mL-min"'. The column temperature was set at 30 "C and detection wavelength was 345 nm. RESULTS: The linear range of luteolin and apigenin were 0.143-0.857 ug(r=0.999 5) and 0.…查看全部>>
王国英;薛培凤;布仁;高建萍
内蒙古医学院药学院,呼和浩特010059内蒙古自治区食品药品检验所,呼和浩特010011内蒙古医学院药学院,呼和浩特010059内蒙古医学院药学院,呼和浩特010059
医药卫生
蒙药蓝盆花木犀草素芹菜素高效液相色谱法含量测定
Mongolian medicine Scabiosa tschilliensis Luteolin Apigenin HPLC Content determination
《中国药房》 2012 (15)
1407-1409,3
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