中国药房2012，Vol.23Issue(15)：1409-1410,2.DOI:10.6039/j.issn.1001-0408.2012.15.26
HPLC法测定星宿菜中山柰酚的含量
Content Determination of Kaempferol in Lysimachia fortunei by HPLC
付远清 1莫逢伟 1廖厚知2
作者信息
- 1. 北海市卫生学校附属医院药剂科,广西北海536100
- 2. 广西汇科药物研究所,南宁530007
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the content determination of kaempferol in Lysimachia fortunei. METHODS: HPLC method was adopted. Shimadzu VP-ODS Cis(150 mm><4.6 mm, 5 um) column was used with mobile phase consisted of acetonitrile-0.2% phosphoric acid solution (42:58) at the flow rate of 1.0 mL'tnuT'. The detection wavelength was set at 370 nm. RESULTS: The linear range of kaempferol ranged from 0.206 2 to 1.031 0 ug(r=0.999 8) with an average recovery of 98.67% (RSD=1.24% ,w=6). CONCLUSION: The method is accurate, sensitive and good in reproducibility. It can be applied for the quality control of L fortunei.关键词
星宿菜/山柰酚/高效液相色谱法/含量测定Key words
Lysimachia fortunei/ Kaempferol/ HPLC/ Content determination分类
医药卫生引用本文复制引用
付远清,莫逢伟,廖厚知..HPLC法测定星宿菜中山柰酚的含量[J].中国药房,2012,23(15):1409-1410,2.
