中国药房2012，Vol.23Issue(21)：1954-1956,3.DOI:10.6039/j.issn.1001-0408.2012.21.14
丁苯酞对大鼠体内硝苯地平药动学的影响研究
Effects of Butylphthalide on the Pharmacokinetics of Nifedipine in Rats
杨秀岭 1郭利 1张志清 1王淑梅 1张如春2
作者信息
- 1. 河北医科大学第二医院药学部,石家庄050000
- 2. 河北益生医药有限公司,石家庄050051
- 折叠
摘要
Abstract
OBJECTIVE: To investigate the effects of butylphthalide on the pharmacokinetics of nifedipine in rats. METHODS: SD rats were randomly divided in control group (nifedipine 10 mg·kg-1) and trial group (nifedipine 10 mg·kg-1+butylphthalide 80 mg·kg-1) with 6 rats in each group. The rats were received relevant drugs intragastrically, and blood samples were collected from fundus venous plexus 0.5 mL each time before treatment and 0.08, 0.25, 0.33, 0.5, 1, 1.5, 2, 2.5, 3, 4, 5 h after treatment. The blood concentration of drugs was determined by HPLC, and the pharmacokinetic parameters were calculated by DAS2.1.1 program. The determination was performed on Diamonsil? C18 column with mobile phase consisted of acetonitrile-water (56:44) at a flow rate of 1.0 mL·rnin-1. The detection wavelength was set at 238 nm. RESULTS: The linear range of nifedipine was 0.079~5.080 μg·mL-1(r=0.998 3)withan average extraction recovery of 75.67%~-81.38%. The RSD of intra-day and inter-day were all lower than 7%. The pharmacokinetic parameters of nifedipine in control group vs. Trail groups were as follows: AUC0-∞ : (8.23 ± 2.23) mg·h·L-1 vs.(4.14±1.63) mg·h·L-1; Cmax: (2.76±0.79) mg·L-1 vs.( 1.85+ 0.55) mg·L-1; t1/2t (2.9± 1.27) h vs.(2.14±0.84) h; CL/F: (2.61 ±0.79) L·h-1·kg-1 vs.(4.96± 1.47) L·h-1·kg-1. There was statistical significance in the difference in AUC0-∞-., Cmax and CL/F between 2 groups (P<0.05). CONCLUSIONS: Butylphthalide can inhibit the absorption of nifedipine in rats.关键词
丁苯酞/硝苯地平/大鼠/药动学/高效液相色谱法Key words
Butylphthalide/ Nifedipine/ Rats/ Pharmacokinetics/ HPLC分类
医药卫生引用本文复制引用
杨秀岭,郭利,张志清,王淑梅,张如春..丁苯酞对大鼠体内硝苯地平药动学的影响研究[J].中国药房,2012,23(21):1954-1956,3.
