中国药房2012，Vol.23Issue(21)：1957-1959,3.DOI:10.6039/j.issn.1001-0408.2012.21.15
HPLC法测定大鼠离体肝微粒体中氯胺酮和去甲氯胺酮的含量及其性别差异研究
Determination of Ketamine and Norketamine in Rats Liver Microsome in Vitro by HPLC and Its Gender Difference
摘要
Abstract
OBJECTIVE; To develop a method for the determination of Icetatnine and norketamine in rat liver microsotne in vitro, and to compare the gender difference of ketamine metabolism in liver microsome between male rat and female rat. METHODS: HPLC method was adopted using phenacetin as internal standard. The determination was performed on Welchrome C18 column with acetonitrile-0.015 mol · L-1 ammonium acetate (pH value adjusted to 5.85 by adding triethylamine, 40:60) as mobile phase at the flow rate of 1 mL·min-1. The detection wavelength was 215 nm, the column temperature was 30 ℃ and the injection volume was 20 μL. The metabolism of 5~1 000 μg·mL-1 ketamince cultured in isolated liver microsome (1.0 mg·mL-1) between male rat and female rat for 15 min was compared. RESULTS: The linear ranges of ketamine were 0.1-20.0 μg·mL-1 (r=0.999 9) and 20.0-1 000.0 μg·mL-1(r=0.999 4). The linear range of norketamine was 0.1~20.0 μg·mL-1(r= 0.999 8). The lowest limit of detection was 2 ng·mL-1. The intra-day and inter-day RSD were all less than 10.8% and 12.7%. And the method recoveries were (89.6 ±8.5)%~(102.5± 6.4)%,(86.5±3.6)%~(101.7 ±4.8)%; extration recoveries were (90.6±6.3)%~(92.8± 4.2)%, (84.5 ± 7.9)%~(87.7 ± 5.5)%. The elimination velocity of ketamine and generation rate of norketamine in male rats microsome were higher than those of female rats in the adding dose of 5-1 000 μg·mL-1. CONCLUSION: The method is sensitive, simple and accurate, and it can be used for the content determination of ketamine and norketamine. The results of velocity indicate the manner of ketamine metabolism in vitro is of gender difference.关键词
高效液相色谱法/氯胺酮/去甲氯胺酮/大鼠/肝微粒体/性别差异/代谢速率Key words
HPLC/ Ketamine/ Norketamine/ Rats/ Liver microsome/ Gender difference/ Metabolism velocity分类
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代晶,杨万清..HPLC法测定大鼠离体肝微粒体中氯胺酮和去甲氯胺酮的含量及其性别差异研究[J].中国药房,2012,23(21):1957-1959,3.
