中国医科大学学报2012，Vol.41Issue(1)：11-13,17,4.
高压氧预处理对脊髓损伤后后角神经元的保护作用
Spinal Cord Posterior Horn Neurons are Protected by Hyperbaric Oxygen Preconditioning after Spinal Cord Injury
摘要
Abstract
Objective To test the hypothesis that spinal cord posterior horn neurons are protected by hyperbaric oxygen preconditioning (HBO-PC) and mediated by inhibition of early apoptosis. Methods 24 Wistar male rats were randomly divided into 2 groups: control group and HBO-PC group. The rats in HBO-PC group were administrated HBO for 5 d,their spinal cords (Tg-Tn) were all transected as same as the control group. At 8 h,l d and 3 d after operation,the spinal cords were cut for coronal frozen sections,and the sections were stained with Nissl and TUNEL staining,they were observed by light microscope and analyzed by statistics. Results Nissl staining demonstrated that the hyperchromic cells were found in posterior horn neurons in two groups at 8 h and 1 d after operation,the hyperchromic cells were lesser in HBO-PC group than that of control group,no big difference between two groups at 3 d postoperative;TUNEL staining demonstrated that the positive cells were most found in posterior horn neurons at 8 h and 1 d after the operation,there were difference between two groups at 3 d postoperative. Statistic analysis demonstrated that the difference was obvious at 8 h (P < 0.05) and significant at 1 d (P < 0.01 )postoperative, respectively. Conclusion The results demonstrated that HBO-PC could protect posterior hom neurons after spinal cord injury, the effects were shown most significant within 1 d after spinal cord injury.关键词
高压氧预处理/脊髓损伤/前角运动神经元/凋亡Key words
hyperbaric oxygen preconditioning/ spinal cord injury/ posterior hom neuron/ apoptosis分类
医药卫生引用本文复制引用
李洪鹏,巴方,白丹,高杰..高压氧预处理对脊髓损伤后后角神经元的保护作用[J].中国医科大学学报,2012,41(1):11-13,17,4.基金项目
国家自然科学基金资助项目(30872669) （30872669）
