中国医科大学学报2012，Vol.41Issue(1)：69-72,76,5.
脑血管病患者高同型半胱氨酸血症发生机制的初步研究
Preliminary Study on the Mechanism of Hyperhomocysteinemia in Cerebrovascular Disease Patients
摘要
Abstract
Objective To investigate the mechanism of hyperhomocysteinemia in cerebrovascular disease patients. Methods Senun Hcy level was measured by enzyme method. Auto chemiluminescence immunoassay (CLIA) was applied for detecting serum folate and vitamin B12 levels in all groups. The gene polymorphism of MTHFR C677T of patients with cerebral infarction (CI),cerebral hemorrhage (CH) and healthy controls were analyzed by a TaqMan real-time PCR technology. Results Serum Hey levels in cerebrovascular disease group were significantly higher than that of control group (P < 0.01). Moreover,serum Hey levels in CI group were obviously higher than that of CH group (P < 0.01). In contrast,serum folate and VitB12 levels in cerebrovascular diseases group were obviously lower than that of control group (P < 0.01). Meanwhile,correlation analysis results showed that serum folate and VitB12 levels were negatively correlated with serum Hey levels in cerebrovascular diseases group (r1=-0.80,r2=-0.83). The genotype distribution of MTHFR C677T and the frequency of T allele had significant differences between cerebrovascular diseases group and control group (P < 0.05). Serum Hey levels in TT genotype of MTHFR C677T were obviously higher than that of CT and CC genotype in all groups (P < 0.01). But there were no significant difference of Berum Hcy levels between CT and CC genotype (P> 0.05). Conclusion Hyperhomocysteinemia was a risk factor resulting in occurrence of cerebrovascular diseases. The mutation of MTHFR C677T and low levels of serum folate and VitB12 were genetic and nutritional factors elevating the level of serum Hcy.关键词
高同型半胱氨酸血症/脑血管病/机制Key words
hyperhomocysteinemia/ cerebrovascular disease/ mechanism分类
医药卫生引用本文复制引用
郑伟,杨智,安殿梅,孟冬娅,胡晓芳..脑血管病患者高同型半胱氨酸血症发生机制的初步研究[J].中国医科大学学报,2012,41(1):69-72,76,5.基金项目
辽宁省科学技术计划课题(2007225004-6) （2007225004-6）
沈阳军区总医院重点基金课题(06Y-Z13) （06Y-Z13）
