中国医科大学学报2012，Vol.41Issue(2)：104-107,4.
环氧合酶2介导姜黄素对慢性脂多糖诱导的脊髓突触可塑性损害的拮抗作用
Cyclooxygenase-2 Mediated Antagonism Effect of Curcumin on Chronic Exposure to LPS-induced Impairment of Spinal Synaptic Plasticity
摘要
Abstract
Objective To explore the role of cyclooxygenase-2 (COX-2) and curcumin on chronic exposure to lipopolysaccharide (LPS)-induced inhibition of spinal long-term potentiation (LTP). Methods Rats were intraperitoneally injected with LPS (3 mg/kg), curcumin (300 mg/kg)and curcumin(300 mg/kg)respectively or NS398,which was a selective inhibitor of COX-2 (10 mg/kg),combined with LPS(3 mg/kg).The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal hom in the lumbar enlargement to observe the effect of curcumin and NS398 on the chronic exposure to LPS-induced inhibition of LTP after 7 days. The expression of COX-2 under different conditions was determined by Western blot method. Results Spinal LTP was significantly attenuated by chronic exposure to LPS and this effect was robustly inhibited by curcumin. But curcumin itself did not affect spinal LTP by tetani. Chronic exposure to LPS-induced inhibition of spinal LTP was reversed by NS398. Elevated expression of COX-2 protein in LPS-treated rats at 7 d after injection was significantly suppressed by NS398 and curcumin respectively. Conclusion Chronic exposure to LPS-induced inhibition of spinal LTP are reversed by curcumin and this effect may be mediated via COX-2.关键词
环氧合酶2/姜黄素/脂多糖/长时程增强/脊髓Key words
cyclooxygenase-2/ curcumin/ lipopolysaccharide/ long-term potentiation/ spinal cord分类
医药卫生引用本文复制引用
杨红卫,王翔宇..环氧合酶2介导姜黄素对慢性脂多糖诱导的脊髓突触可塑性损害的拮抗作用[J].中国医科大学学报,2012,41(2):104-107,4.基金项目
国家自然科学基金资助项目(30970930) （30970930）
宜昌市科学技术研究与开发项目(A09301-24) （A09301-24）
三峡大学人才科研启动基金资助项目(KJ2009B010) （KJ2009B010）
