中国医科大学学报2012，Vol.41Issue(2)：131-134,138,5.
CXCR4在不同转移潜能肝癌细胞中的表达及意义
The Expression and Significance of CXCR4 in Human Liver Cancer Cell Lines with Different Metastatic Potentials
王勃 1李少林 1张俊 1胡敏 1袁犁1
作者信息
- 1. 重庆医科大学放射医学教研室,重庆400016
- 折叠
摘要
Abstract
Objective To detect the expression of CXCR4 in human liver cancer cell lines with different metastatic potentials and to investigate the effect of CXCR4 on proliferation and metastasis of human liver cancer. Methods Matrigel invasion assays were applied to screen cell subclones of human liver cancer cell lines (3B) with different metastatic potentials,and transwell assays were applied to observe the cell motility and invasiveness for verifying the above result. RT-PCR and Western blot were used to detect the gene and protein expression of CXCR4 in cell subclones with different metastatic potentials. CCK-8 assay was applied to detect cell growth curve and doubling tune;flow cytometry was used to test cell cycle and apoptosis rate. Results Two cell subclones with high metastatic potential and low metastatic potential were obtained through invasion assays,and it was verified that invasion ability in cell subclones with high metastatic potential is greater than that in cell subclones with low-metastatic potential by transwell test. The expression level of CXCR4 in mRNA and protein level in cell subclones with high metastatic potential were significantly higher than those in cell subclones with low metastatic potential. There were significant differences between both cell subclones in cell proliferation,doubling time,cell cycle and apoptosis rate. Conclusion Overexpression of CXCR4 is highly related to metastatic potential of human liver cancer cells. CXCR4 may play an important role in malignant progression of human liver cancer. It may be an important predictor and show us some new therapeutic approaches to beat liver cancer.关键词
肝癌/趋化因子受体/CXCR4/Transwell/侵袭/转移Key words
liver cancer/ chemokine receptors/ invasion/ metastasis分类
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王勃,李少林,张俊,胡敏,袁犁..CXCR4在不同转移潜能肝癌细胞中的表达及意义[J].中国医科大学学报,2012,41(2):131-134,138,5.
