中国医科大学学报2012，Vol.41Issue(2)：148-150,157,4.
神经母细胞瘤恶性进展相关基因初步筛选及验证
Screening and Identification of Candidate Genes Associated with Progression of Neuroblastoma
摘要
Abstract
Objective To discover the molecular mechanisms underlying progression of human neuroblastoma by screening the candidate genes in clinical specimens. Methods The candidate genes mapped on human chromosome 17q gain,which was significantly associated with the neuroblastoma,were screened by combining the array-CGH results with expression profiling,and identified by bioinformatic analysis and RT-PCR. Results A novel gene linked to progression of neuroblastoma named ncKAN was found on chromosome 17q gain,which included two transcripts,Nblal0727 and Nbla 12061 .and differential expression can be detected in various tissues and neuroblastoma cell lines with a single copy and amplification of MYCN. Furthermore,high expression of ncRAN was associated with poor prognosis (P =0.000 221). Conclusion The novel gene mapped on chromosome 17q gain may be an oncogene which is associated with progression of neuroblastoma.关键词
神经母细胞瘤/17号染色体/癌基因/cDNA芯片Key words
neuroblastoma/ chromosome 17/ oncogene/ cDNA chip分类
医药卫生引用本文复制引用
于萌,朱育焱,杨春明,王海波,李元元,中川原章,郑志红..神经母细胞瘤恶性进展相关基因初步筛选及验证[J].中国医科大学学报,2012,41(2):148-150,157,4.基金项目
国家自然科学基金资助项目(31000572),教育部新教师基金资助项目(20102104120013).辽宁省博士科研启动基金资助项目(20101148),辽宁省教育厅高校科研计划(L2010622) （31000572）
