中国医科大学学报2012，Vol.41Issue(3)：197-200,4.
甲基强的松龙对脊髓半横断大鼠腓肠肌乙酰胆碱酯酶表达的影响
Effects of Methylprednisolone on Acetylcholinesterase in Motor Endplate after Spinal Cord Semi-transected in Rats
摘要
Abstract
Objective To study the effects of methylprednisolone (MP) on acetylcholinesterase (AChE) in gastrocnemius neuromuscular junction after spinal cord injury in rats. Methods Ninety-six 10-week-old SPF Wistar male rats were randomly divided into 3 groups,including sham operation group (A group,n =16),spinal cord injury group (B group,n =40) and MP treated group (C group,n =40) after spinal cord injury. B,C group received the right spinal cord semi-transection,and MP intervention was given to C group after surgery;right leg gastrocnemius were collected on dl ,d3,wl ,w2 and w4 for frozen sections. The expression of AChE was detected by Copper-iron cyanide improved staining. We measured the average optical density of specimens for positive expression area of each group. Results The gastrocnemius muscle AChE expression was stained as brown; Id after spinal cord semi-transection operation,,there was no significant difference on the average optical density value of rat gastrocnemius muscle AChE stained area from 3 different groups;3 d after operation,no significant difference was observed on the average optical density of AChE staining between B and C group,but lower than group A; 1 week,2 week after operation,the mean optical density value of AChE staining area in group B was lower than group Cboth B and C group were lower than group A;4 week after operation,AChE staining in group C was restored,with no significant difference in group A;group B remained at low levels which was lower than A, C group. Conclusion Spinal cord injury induced AChE reduction in the level of the neuromuscular junction , MP early intervention helps to skeletal neuromuscular recovery of AChE expression.关键词
甲基强的松龙/运动终板/乙酰胆碱酯酶/大鼠Key words
methylprednisolone/ motor endplate/ acetylcholinesterase/ rat分类
医药卫生引用本文复制引用
高杰,巴方,李洪鹏..甲基强的松龙对脊髓半横断大鼠腓肠肌乙酰胆碱酯酶表达的影响[J].中国医科大学学报,2012,41(3):197-200,4.基金项目
国家自然科学基金资助项目(30872669) （30872669）
