中国医科大学学报2012，Vol.41Issue(3)：220-223,4.
RhoA/ROCK信号系统对血管外膜炎症介导的大鼠颈动脉平滑肌细胞增殖和表型变化的影响
Effects of RhoA/ROCK Signaling Pathway on the Proliferation and Phenotypic Modulation of Vascular Smooth Muscle Cells in Perivascular Inflammatory Injured Rat Carotid Artery
程木秋 1霍海洋 1张海山1
作者信息
- 1. 中国医科大学附属第一医院心内科,沈阳110001
- 折叠
摘要
Abstract
Objective To investigate the role of RhoA/ROCK signaling pathway in the proliferation and phenotypic modulation of vascular smooth muscle cells in peri vascular inflammatory injured rat carotid artery. Methods A total of 20 Wistar rats were randomly divided into 2 groups as model group and control group. Bilateral carotid artery model with chronic inflammatory injury was established by silicone collar which was positioned around the carotid artery adventitia of the rat. After operation,the rats were fed with standard food and free water for 7 days. Carotid artery specimens was obtained for HE staining to evaluate the vascular morphological changes. Western blot was used to detect the expression of vascular tissue SM a-actin and ROCK2 protein; RT-PCR method was applied to detect mRNA expression of vascular tissue SM a-actin and R0CK2. Results Compared with the carotid artery of control rats,the thicker adventitia and adventitial inflammation was observed in the model group;the intima smooth muscle cells significantly proliferated and arranged in disorder. Compared with control group,carotid artery vascular smooth muscle cell SM in model group had higher a-actin expression at both mRNA and protein level;no significant difference of R0CK2 mRNA expression was observed between 2 groups,however,the protein expression was significant higher in model group. Conclusion RhoA / ROCK signaling pathway may be involved in the proliferation and phenotypic modulation of vascular smooth muscle cells in perivascular inflammatory injured rat carotid artery.关键词
血管外膜/硅胶管/颈动脉损伤/RhoA/ROCK信号转导通路Key words
vascular adventitia/ silicone collar/ carotid artery injury/ RhoA / ROCK signaling pathway分类
医药卫生引用本文复制引用
程木秋,霍海洋,张海山..RhoA/ROCK信号系统对血管外膜炎症介导的大鼠颈动脉平滑肌细胞增殖和表型变化的影响[J].中国医科大学学报,2012,41(3):220-223,4.
