中国医科大学学报2012，Vol.41Issue(3)：237-240,4.
二次离心法制备富血小板血浆离心条件的比较
Effects of Different Platelet-rich Plasma Preparation Methods on the Concentration of Platelet
摘要
Abstract
Objective To explore an ideal method for FRP concentration by double centrifugation. Methods Totally 2.7 milliliters whole blood was collected from central ear artery of the albino rabbit,and centrifuged according to different conditions (Speed: 1 000-2 000 r/min; time:5-20 min). Calculating of the platelet concentration and recovery percent of platelet were performed. Results Several centrifuge conditions successfully prepared PRP. Initial centrifugation at 1 400 r/min for 10 min,which is the critical condition for the formation of Albug-inea,achieved highest rate of platelet recovery,and the operation of upper plasma is simple. Second centrifuge condition was set for 1 400 r/min >10 min. Platelet counts in PRP prepared by four different methods were increased with respect to venous blood platelet counts. Percent recovery of platelet by the second method was observed to be significantly higher when compared to other methods. Conclusion Double cen-triiugation is an efficient method for PRP preparation. Initial centrifugation conditions at speed of 1 400 r/min for 10 min and followed with another centrifugation at 1 400 r/min (> 10 minutes) has the higher platelet count and percent recovery of platelet. It could be an efficient method for PRP preparation.关键词
富血小板血浆/制备Key words
platelet-rich plasma/ preparation分类
医药卫生引用本文复制引用
金哲,吕刚,王禹祥..二次离心法制备富血小板血浆离心条件的比较[J].中国医科大学学报,2012,41(3):237-240,4.基金项目
辽宁省博士科研启动基金(20101137) （20101137）
