中国药理学通报2012，Vol.28Issue(4)：473-477,5.DOI:10.3969/j.issn.1001-1978.2012.04.008
瓜子金皂苷己对MPP+诱导PC12细胞凋亡的保护作用
The protective effect of polygalasaponin F against MPP + induced PC12 cellular apoptosis
摘要
Abstract
Aim To observe the effects and mechanism of polygalasaponin F( PS-F) on PC12 cellular apopto-sis induced by 1 -methyl-4-phenylpyridinium ion ( MPP+). Methods MTT assay was used to measure the viability of the PC 12 cells and flow cytometry was used to analyze the apoptotic rate of PC 12 cells. The expression of active Caspase-3 in PC 12 cells was detected by Western blot. In addition, the mitochondrial membrane potential was determined by JC-1 staining inverted microscope. Results When exposed to 500 μmol ? L-1 MPP + for 48 h, the viability and the mitochondrial membrane potential of PC 12 cells decreased significantly, and the apoptosis rate and the expressionof active Caspase-3 were increased significantly. Treatment with PS-F, the cell viability (P < 0. 01) and the MMP were significantly increased, the apoptosis rate and the expression of active Caspase-3 ( P < 0. 01 ) were significantly decreased compared with MPP + group. Conclusion PS-F protects PC 12 cells against apoptosis induced by MPP + and the mechanism may be related to ameliorate the mitochondrial dysfunction and decrease the expression of active Caspase-3.关键词
帕金森病/瓜子金皂苷己/1-甲基-4-苯基-吡啶离子/PC12细胞/细胞凋亡/线粒体Key words
Parkinson's diseases/ polygalasaponin F/ l-methyl-4-phenyl-pyridinium/ PC12 cells/ cellular apoptpsis/ mitochondrion分类
医药卫生引用本文复制引用
吴苗苗,苑玉和,胡金凤,楚世峰,何鑫,陈乃宏..瓜子金皂苷己对MPP+诱导PC12细胞凋亡的保护作用[J].中国药理学通报,2012,28(4):473-477,5.基金项目
国家自然科学基金资助项目(No U832008,30973887,90713045,81073130,81072541),科技部国际合作项目(No 2010DFB32900),国家科技重大专项(No2012ZX09301002-004,2012ZX09103101-006) （No U832008,30973887,90713045,81073130,81072541）
