中国药理学通报2012，Vol.28Issue(4)：490-495,6.DOI:10.3969/j.issn.1001-1978.2012.04.012
甲基莲心碱对2型糖尿病模型大鼠作用的代谢组学研究
Effects of neferine on type 2 diabetic mellitus rats by metabonomics method
摘要
Abstract
Aim To study whether neferine influences urinary metabolites of type 2 diabetic mellitus rats. Methods The rats were fed with high-sucrose and high-fat chow for 4 weeks, then were injected intraper-itoneal(ip) with STZ (30 mg ? kg-1). One week later, rats with fasting blood sugar levels higher than 7. 8 mmol ? L-1 were defined as type 2 diabetes mellitus (T2DM) rats. Neferine treated T2DM rats were injected intraperitoneal (ip) with neferine (20 mg ? kg-1? d-1) for 4 weeks. Fasting blood sugar of all rats was measured after 4 weeks. 24 h urine samples from rats were collected and prepared. All the separations were performed on UPLC/QTOF-MS system. Results The blood glucose values in neferine treated T2DM ratswere decreased in comparison with those in T2DM rats (P < 0. 05). Dihydroxyfumaric acid in urinary metabolite profiles of neferine treated T2DM rats was decreased compared with that in T2DM group. Increase of PGI2, LXA4 and LXB4 was revealed in the urinary metabolite profiles of neferine treated T2DM group than that in T2DM group. Conclusions Neferine can decrease blood glucose and change urinary metabolite profiles of T2DM rats. The mechanism of neferine may be associated with antioxidant stress and anti-inflammatory effect.关键词
甲基莲心碱/2型糖尿病/代谢组学/链尿佐菌素/大鼠模型/液质联用Key words
neferine/ type 2 diabetes mellitus/ metabo-nomics/ streptozocin/ rat model/ LC/GC-MS分类
医药卫生引用本文复制引用
刘双梅,李国栋,梁尚栋,李桂林,汤晓丽,徐宏,林加日,谢金燕,刘俊,孔繁君,吴饶平..甲基莲心碱对2型糖尿病模型大鼠作用的代谢组学研究[J].中国药理学通报,2012,28(4):490-495,6.基金项目
国家自然科学基金资助课题(No 31060139,30860086,30660059) （No 31060139,30860086,30660059）
江西省科技支撑计划-社会发展支撑计划重点项目(No 2010BSA09500,2011BBG70009-1) （No 2010BSA09500,2011BBG70009-1）
江西省教育厅科学技术研究项目(No赣教技字[09674]号) （No赣教技字[09674]号）
