中国药理学通报2012，Vol.28Issue(4)：496-500,5.DOI:10.3969/j.issn.1001-1978.2012.04.013
丹酚酸B对血管内皮细胞迁移及管腔形成的影响
Effect of Sal B on angiogenesis and tube formation of vascular endothelial cells
摘要
Abstract
Aim To observe the effect of Sal B on proliferation , migration and tube formation in vitro, discuss the influence on the neovascularization and find out the mechanism. Methods Tritium-labeled thymidine (3 H-TdR) method was used to determine the effect of Sal B on the proliferation of HUVECS. HUVECS migration was detected by Transwell assay. Capillary was detected by Matrigel-plug assay in vitro. Enzyme-linked im-munosorbent assay (ELISA) and Western blot method were used to determine the effect of Sal B on the content of VEGF secreted by HUVECS and the expression of VEGF, respectively. Results 3H-TdR assay showed that Sal B promoted the proliferation of HU-VECS. The Transwell assay showed that Sal B accelerated the migration of HUVECS. The tube formation assay showed that the tube number and the length of the tube were higher than control group. After the HUVECS were treated by Sal B, the concentrations of VEGF were higher in cellular supernatant and the expression of VEGF was significantly up-regulated compared with the control group. Conclusion Sal B promotes the proliferation, migration and tube-like structure formation. Its mechanism may be related to the up-regulation of VEGF expression.关键词
丹酚酸B/人脐静脉内皮细胞/血管新生/3H参入法/Transwell,管腔形成Key words
Sal B/ HUVECS/ angiogenesis/ tritium-labeled thymidine/Transwell/tube formation分类
医药卫生引用本文复制引用
许波华,沈祥春,张良,袁冬平,龙军,喻斌,许立..丹酚酸B对血管内皮细胞迁移及管腔形成的影响[J].中国药理学通报,2012,28(4):496-500,5.基金项目
国家"十二五"重大新药创制科技重大专项(No 2011ZX09102-002-07) （No 2011ZX09102-002-07）
