中国药理学通报2012，Vol.28Issue(4)：526-530,5.DOI:10.3969/j.issn.1001-1978.2012.04.020
柚皮苷调控心肌PPARγ表达对实验性2型糖尿病心肌病大鼠模型心肌损伤的防治作用
Protective effects of Naringin on experimental rats model of Type 2 diabetic cardiomyopathy via regulation of PPARγ signaling
摘要
Abstract
Aim To explore the protective effects of Naringin on myocardial structure function with STZ-in-duced diabetic cardiomyopathy in rats via regulation of peroxisome proliferation factor receptor -y ( PPAR-y ). Methods The expression of PPAR7 was tested by im-munohistochemical method. The expression of BNP and cardiac troponin I(cTnl) were detected by quantitative immunofluorescence. The myocardial cell ultrastructure was tested by transmission electron microscope. Results ① The enhanced PPAR7 expression was prominently stained in endothelial cells and myocardial cell cytoplasm area. Compared with the normal control group, OGTT was positive. The level of heart/body weigh, FFA,HOMA-IR,GSP and BNP increased, The Myocardial cell ultrastructure was injured in diabetes cardiomyopathy model control group. ② Compared with the model control group, the enhanced PPAR7 expres-sion was downregulated, PPAR-y positive vascular density weakened and the corresponding gray value in-creasesd(P <0. 05) ,The injury in the myocardial cell ultrastructure and OGTT ameliorated. The level of heart/body weigh, FFA, HOMA-IR, GSP and BNP decreased in a moderate or high dose of Naringin and Pio-glitazone treatment groups. ③ Compared with the Piogl-itazone treatment group, the level of decrease was better in BNP but poorer in HOMA-IR in a high dose of Naringin treatment group. Conclusion Naringin can ease the injury in the myocardial cell ultrastructure. Its mechanisms may be associated with regulating myocardial PPAR7 expression mediated improvement of insulin resistance, block activation of FFA and inhibiting BNP.关键词
糖尿病心肌病/过氧化物酶体增殖因子活化受体γ/柚皮苷/B型脑钠肽/心肌超微结构/葡萄糖耐量试验Key words
diabetic cardiomyopathy/ PPAR-y/ Naringin / BNP/ myocardial ultrastructure/ OGTT分类
医药卫生引用本文复制引用
吴铿,游琼,黄瑞娜,涂焰明,梁建光..柚皮苷调控心肌PPARγ表达对实验性2型糖尿病心肌病大鼠模型心肌损伤的防治作用[J].中国药理学通报,2012,28(4):526-530,5.基金项目
广东省自然科学基金资助项目(No s2011010002620) （No s2011010002620）
