中国药理学通报2012，Vol.28Issue(6)：764-768,5.DOI:10.3969/j.issn.1001-1978.2012.06.008
溶血磷酯酸诱导白细胞介素13受体α2mRNA表达并抑制胶原蛋白合成
LPA-induced expression of IL-13Rα2 and inhibitory effect of LPA on synthesis of collagen
摘要
Abstract
Aim To study the effect of lysophosphatid-ic acid (LPA) on interleukin 13 receptor α2 (IL-13Rα2) gene expression and collagen type I(COLA I) synthesis in lung fibroblasts. Methods Cell culture was used. The growth of lung fibroblasts HFL-1 cells was observed with microscope. HFL-1 cells were stimulated with LPA in different concentrations. The expression of IL-13Rα2 mRNA and COLA I was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR). The expression of COLA I protein was evaluated by immunohistochemisty. Electrophore-sis strip and immunohistochemical image were analysed by image analysis. All results were processed by statistics. Results ① LPA enhanced the expression of IL-13Rα2 mRNA in HFL-1 cells in a time-dependent manner. ② With the increasing of the concentration ofLPA, IL-13 Rα2 mRNA expression significantly increased. After 1 μmol·L-1 LPA stimulated HFL-1 cells, IL-13 Rα2 mRNA expression reached peak. ③ LPA had no effect on IL-13Rαl expression of HFL-1. ④ COLA I expression of HFL-1 cells was reduced with LPA stimulation. The expression of COLA I mRNA was increased with IL-13. The expression of COLA I mRNA was higher in LPA + IL-13 group than LPA group and lower than IL-13 group.⑤ Immunohistochemical results and RT-PCR results were consistent. Conclusion LPA induces the expression of IL-13 Rα2 mRNA and downregulates the synthesis of COLA1 in lung fibroblasts.关键词
溶血磷脂酸/人肺成纤维细胞/白细胞介素13受体α 2/Ⅰ型胶原/纤维化/抑制性受体/白细胞介素13Key words
lysophosphatidic acid/ human lung fibro-blast/ interleukin 13 receptor α2/ collagen type I / fi-brosis/ inhibiting receptor/ interleukin 13分类
医药卫生引用本文复制引用
石小玉,何晓璐,夏小春,熊丽霞,赵林,李文林..溶血磷酯酸诱导白细胞介素13受体α2mRNA表达并抑制胶原蛋白合成[J].中国药理学通报,2012,28(6):764-768,5.基金项目
国家自然科学基金资助项目(No 30860118) （No 30860118）
江西省卫生厅科研项目(No 20062016) （No 20062016）
江西省教育厅科技项目(赣教技字[2007] 81号) （赣教技字[2007] 81号）
