中国药理学通报2012，Vol.28Issue(6)：833-838,6.DOI:10.3969/j.issn.1001-1978.2012.06.023
EGb761对NMB诱导的小鼠妊娠子宫平滑肌细胞中NF-κB活性和 IL-6表达的影响
The effects of EGb761 on the activity of NF-κBp65 and expression of IL-6 induced by NMB in pregnant primary cultured smooth muscle cells
摘要
Abstract
Aim To investigate the effects of Ginkgo biloba extract 761 (EGb761) on the activity of nuclear factor κB p65 (NF-κBp65) and expression of interleu-kin-6 (IL-6) induced by Neuromedin B(NMB) in primary cultured smooth muscle cells from the term. Methods The primary cultured smooth muscle cells with positive expression of NMB receptor (NMBR) from the term were prepared. The combination of NMBR and NF-κBp65 RNA interference (RNAi), real time PCR and Phosphorylation ELISA methods were used to study the effects of EGb761 on the activity of NF-κBp65 and expression of IL-6 induced by NMB in smooth muscle cells. Results The NF-κBp65 DNA binding activity was significantly lower in EGb761 group with high concentration (100 mg · L-1) than that in control group and low dose concentration group (P<0.01). However, there were not differencescompared low or middle concentration groups with control group. The pretreated with high concentration of EGb761 could significantly reduce NF-κBp65 activity and IL-6 expression induced by NMB in pregnant smooth muscle cells (P < 0. 01), and these changes were significantly correlated (r = 0. 892, P <0. 01). Besides, the inhibiting role of EGb761 could be blocked by NMBR and NF-κB RNAi, respectively. Moreover, the blocking efficiency of two genes knockdown showed no significant difference. Conclusion EGb761 can inhibit NF-κBp65 activity and expression of IL-6 via NMBR pathway in primary cultured smooth muscle cells from the term.关键词
银杏叶提取物/神经调节素B/核因子κB/白介素6/子宫平滑肌细胞/原代培养/RNA干扰Key words
ginkgo biloba extract/ neuromedin B receptor / nuclear factor kappa B/ interleukin-6/ myome-trial smooth muscle cells/primary culture/ RNA interference分类
医药卫生引用本文复制引用
张卫社,谢志萍,吴梅婷,费奎琳,梁清华..EGb761对NMB诱导的小鼠妊娠子宫平滑肌细胞中NF-κB活性和 IL-6表达的影响[J].中国药理学通报,2012,28(6):833-838,6.基金项目
国家自然科学基金资助项目(No 30672241) （No 30672241）
中国博士后基金资助项目(No 20070410312) （No 20070410312）
