中国药理学通报2012，Vol.28Issue(6)：839-842,4.DOI:10.3969/j.issn.1001-1978.2012.06.024
黄芩苷PC12细胞跨膜转运的实验研究
Transport behavior of baicalin in PC12 cells
摘要
Abstract
Aim To investigate the transport behavior of baicalin in PC12 cells and to explain the material basement of nervous system protection activity of baicalin. Methods PC12 cells were used as for research, and HPLC and MS were applied to detect the transport behavior of baicalin. Results When cells were treated by baicalin of 0. 1 mg·L-1, the concentration of baicalin in cells reached a peak when incubating for 30 min and there was no baicalin left after 8 h; when the dosage of baicalin from 0.05 mg·L-1 to 0. 4 mg·L-1 was in administration, intracellular baicalin concentration and administration dosage showed linear positive correlation; verapamil and nimodipine had effect on transport; in addition, baicalein and 6-methoxy-baica-lin could be detected in the cells. Conclusion Baicalin can transport into PC12 cells and can be affected by some inhibitors, producing compounds.关键词
黄芩苷/跨膜转运/PC12细胞/黄芩素/6-甲氧基-黄芩苷/P-糖蛋白Key words
baicalin/ transmembrane transport/ PC12 cells/ baicalein/ 6-methoxy-baicalin/ P-glycoprotein分类
医药卫生引用本文复制引用
赵爽,雷帆,李慧颖,王玉刚,柴玉爽,杜力军..黄芩苷PC12细胞跨膜转运的实验研究[J].中国药理学通报,2012,28(6):839-842,4.基金项目
国家自然科学基金资助项目(No 30973896,30801523,81073092),"重大新药创制"国家科技重大专项资助项目(No 2012ZX09102201-008,2012ZX09103201-041) （No 30973896,30801523,81073092）
