中国药理学通报2012，Vol.28Issue(7)：897-900,4.DOI:10.3969/j.issn.1001-1978.2012.07.003
细胞培养稳定同位素标记技术的发展及其在脑病研究中的应用
Development of stable isotope labeling technique and its application in encephalopathy research
摘要
Abstract
It is crucial to find out the brain specific protein mark - ers for clinical diagnosis and drug mechanism . Human brain is a complex organism, so the single-target, non-dynamic and local method is not suitable for brain research. The development of quantitative proteomics, which is based on the mass spectrum, greatly accelerates the research process. As the accurate quanti -tative method of protein, the stable isotope labeling with aminoacids in cell culture (SILAC) is a system, dynamic technology. It can provide new ideas for the mechanism research and the treatment strategy of traditional Chinese medicine in encephalop -αthy.关键词
定量蛋白质组学/SILAC/脑病/神经血管单元/中药/网络药理学Key words
quantitative proteomics / SILAC/ encephalopathy / neurovascular unit/ traditional Chinese medicine / network pharmacology分类
医药卫生引用本文复制引用
朱丽,刘安,杨洪军,陈畅,李德凤,吴传鸿,高健,李韶菁..细胞培养稳定同位素标记技术的发展及其在脑病研究中的应用[J].中国药理学通报,2012,28(7):897-900,4.基金项目
国家自然科学基金资助项目(No 30901976) （No 30901976）
国家十二五"重大新药创制"科技重大专项资助项目(No 2012ZX09103-201-055) （No 2012ZX09103-201-055）
