中国药理学通报2012，Vol.28Issue(7)：911-916,6.DOI:10.3969/j.issn.1001-1978.2012.07.007
PHⅡ-7通过升高ROS诱导K562和K562/A02凋亡
PHⅡ-7 induces apoptosis on K562 and K562/A02 by increasing ROS production
摘要
Abstract
Aim To investigate the effect of PH II -7 on the cytotoxic effects of K562 and K562/A02 and its related mechanism. Methods Cell viability was detected by WST-8 assay; apoptosis analysis was performed by apoptosis detection kit and flow cytometry ; ROS detection was also completed by flow cytometry. Confocal microscope and Western blot analysis was used to de -tect the changes after treatment with PH II-7. Results PH II -7 had almost equally cytotoxic effect on K562 and K562/A02 cell lines. Elevated ROS production was observed in both the two cell lines after treatment with PH II -7. PH II -7 could induce apoptosis on K562 and K562/A02, and the apoptotic rate was significant -ly reduced when coincubated with 5 mmol· L NAC. Confocal microscope observation found conspicuous morphological changes after treatment of PH II -7, furthermore , Western bolt analysis confirmed that coincu -bation with NAC could greatly attenuate the cleavage of PARP4, caspase-3 and caspase-9 induced by PH II-7. Conclusion PH II-7 can achieve its cytotoxic effect on K562 and K562/A02 cells by apoptosis induction , and this effect is likely performed by increas -ing ROS production within the cells.关键词
PHⅡ-7/活性氧/凋亡/NAC/P-gp/CMLKey words
PH II-7/ ROS/ apoptosis/ NAC/ P-gp/ CML分类
医药卫生引用本文复制引用
彭洪薇,袁向飞,李真真,颜次慧,李双静,张砚君..PHⅡ-7通过升高ROS诱导K562和K562/A02凋亡[J].中国药理学通报,2012,28(7):911-916,6.基金项目
国家自然科学基金资助项目(No 30873091,30971291) （No 30873091,30971291）
