中国药理学通报2012，Vol.28Issue(7)：916-920,5.DOI:10.3969/j.issn.1001-1978.2012.07.008
神经肽Y和5-羟色胺在腹泻型肠易激综合征模型大鼠中表达的研究
Expression of NPY and 5-HT in rat model of diarrhea-predominant irritable bowel syndrome
摘要
Abstract
Aim To investigate expression of neu-ropeptide Y(NPY) and 5-hydroxy tryptamine (5-HT) in diarrhea-predominant irritable bowel syndrome ( D- IBS ) and provide theoretical basis for mechanism of D -IBS from the aspect of brain-gut axis. Method A rat model of D-IBS was established with the use of separa -tion of breast milk + stimulation of acetic acid + constraint of the four limbs. Expression of 5-HT protein was detected in colon of rat with immunohistochemistry and expression of NPY mRNA was detected in colon and hypothalamus of rat with real-time fluorescence quantitative reverse transcription polymerase chain re -action ( RT-PCR ). Result Compared with normal group, expression of NPY mRNA were significantly de -creased in colon and hypothalamus of D-IBS model group (P<0. 05). In addition, 5-HT protein expression was markedly increased in colon of D -IBS model group (P<0. 05). Conclusion NPY and 5-HT may be involved in regulatory mechanism of D -IBS.关键词
神经肽Y/5-羟色胺/腹泻型肠易激综合征/脑肠轴/实时荧光定量PCR/下丘脑/结肠Key words
neuropeptider Y/ 5-hydroxy tryptamine/ diarrhea-predominant irritable bowed syndrome / brain-gut axis/ reverse transcription polymerase chain reaction/ hypothalamus / colon分类
医药卫生引用本文复制引用
唐洪梅,房财富,廖小红,李得堂,姚楠,何嘉仑..神经肽Y和5-羟色胺在腹泻型肠易激综合征模型大鼠中表达的研究[J].中国药理学通报,2012,28(7):916-920,5.基金项目
国家自然科学基金资助项目(No 81073160) （No 81073160）
广东省自然科学基金资助项目(No 10151040701000041) （No 10151040701000041）
广州市科技局重大科技生物专项资助项目(No 2008AJ-E4101-6) （No 2008AJ-E4101-6）
广东省科技厅科技资助项目(No 2010B030700065) （No 2010B030700065）
