中国药理学通报2012，Vol.28Issue(7)：930-933,4.DOI:10.3969/j.issn.1001-1978.2012.07.011
脂联素对大鼠缺血/再灌注心肌细胞凋亡及相关蛋白表达的影响
Effects of adiponectin on the cardiomyocyte apoptosis in rats induced by ischemia and reperfusion and the expression of the related protein
摘要
Abstract
Aim To study the effect of adiponectin (APN) on cardiomyocyte apoptosis and the expression of Bcl-2, Bax, Caspase-3 in rats with ischemia/reper-fusion( I/R). Methods The I/R injury model was established by occluding the left anterior descending branch of coronary artery for 30 min and removing the ligation later to reperfusion for 3 hours. Forty-eight rats were divided into 3 groups randomly ; sham group, I/R group and APN + I/R group. The infarct size was determined by using Evens blue and TTC double dye stai -ning in 8 rats in each group. Others were monitored hemodynamics , then the ultrastructural change of myo -cardial tissue was observed by transmission electron mi -croscope; cardiomyocyte apoptosis was marked by TUNEL; Bcl-2, Bax, Caspase-3 protein expressionwere tested by Western blot. Results APN pretreat-ment could decrease infarct size and ameliorate hemo -dynamics of I/R. APN also could inhibit cardiomyo - cyte apoptosis , increase the Bcl-2 protein expression ( P < 0. 05 ) and reduce the Bax and Caspase -3 protein expression (P < 0. 05) in rats with myocardial ische-mia/reperfusion. Conclusion The preexposure to APN provides protective effect on I/R injury, which is likely associated with the inhibition of cardiomyocyte apoptosis by up regulating Bcl-2 protein expression and down regulating Bax, Caspase-3 protein expression.关键词
脂联素/缺血/再灌注损伤/心肌细胞凋亡/Bcl-2/Bax/Caspase-3/TUNELKey words
adiponectin/ ischemia/reperfusion injury/ cardiomyocyte apoptosis/ Bcl-2/ Bax/ Caspase-3/ TUNEL分类
医药卫生引用本文复制引用
郭佳,肖传实,白瑞,边云飞..脂联素对大鼠缺血/再灌注心肌细胞凋亡及相关蛋白表达的影响[J].中国药理学通报,2012,28(7):930-933,4.基金项目
国家自然科学基金资助课题(No 81170198) （No 81170198）
