中国药理学通报2012，Vol.28Issue(7)：938-943,6.DOI:10.3969/j.issn.1001-1978.2012.07.013
低分子量肝素联合吉西他滨对乳腺癌细胞侵袭和迁移能力的影响
Effects of low molecular weight heparin combined with gemcitabine on the invasion and migration of breast cancer cells
摘要
Abstract
Aim To investigate the anti-tumor effects of low molecular weight heparin ( LMWH ) combined with gemcitabine ( GEM ) , and to discuss the related mechanism. Methods Cells were divided into control group, LMWH group, GEM group and combined group. MTT assay was applied to evaluate the viability of MDA-MIB-231 and MDA-MB-435 breast cancer cells. Wound healing assay and Transwell migration assay were performed to assess the effects of drugs on migration, and Transwell invasion assay was employed to evaluate the effects of drugs on invasion of breast cancer cells. The expression of matrix metalloprotein-αse-9(MMP-9) protein was analyzed by Western blot. ELISA was used to determine the expression of hepara -nase(HPA) in the culture medium. Results LMWHdid not exhibit marked effect on the proliferation of MDA-MB-231 and MDA-MB-435 cells, yet it could not enhance the inhibitory effect of GEM on proliferation of the cells. LMWH combined with GEM resulted in enhanced inhibitory effect on migration and invasion compared with the individual use of GEM in MDA -MB- 231 and MDA-MB-435 cells. At the same time, LMWH enhanced the down regulation of MMP-9 and HPA expression induced by GEM. Conclusion LMWH can enhance the inhibitory effects of GEM on migration and invasion in breast cancer cells , which may be correlated to the down regulation of MMP-9 and HPA.关键词
乳腺癌/低分子量肝素/吉西他滨/侵袭/迁移/MMP-9/HPAKey words
breast cancer/ low molecular weight heparin / gemcitabine / invasion/ migration/ MMP-9/ HPA分类
医药卫生引用本文复制引用
赵素容,刘浩,吴成柱,王秀,梁颖,陈超,蒋志文..低分子量肝素联合吉西他滨对乳腺癌细胞侵袭和迁移能力的影响[J].中国药理学通报,2012,28(7):938-943,6.基金项目
国家自然科学基金资助项目(No 81000992,81072207) （No 81000992,81072207）
安徽省高校省级优秀青年人才基金资助项目(No 2010SQRL122) （No 2010SQRL122）
安徽省高等学校省级自然科学研究项目(No KJ2012A202) （No KJ2012A202）
