中国药理学通报2012，Vol.28Issue(7)：970-974,5.DOI:10.3969/j.issn.1001-1978.2012.07.020
知母皂苷对脂多糖引起星形胶质细胞炎症因子释放的影响及机制
Effect of SAaB on the release of inflammatory factors induced by lipopolysaccharide in cultured astrocytes in rats
摘要
Abstract
Aim To investigate whether Saponins from Anemarrhena asphdeloids Bge (SAaB) can protect cortical astrocytes from lipopolysaccharide ( LPS ) induced release of inflammatory factors and to study the mechanisms responsible for this protective effect. Methods Cultured astrocytes were treated with LPS(10 mg· L ) for 24 h, in the absence or presence of SAaB (1,10 and 100 μmol· L-1 ) or the specific inhibitor of JNK SP600125 (10 μmol· L-l) , and harvested. Tumor necrosis factor -α ( TNF-α) and nitric oxide ( NO) generation were analysed respectively by ELISA and Griess technology. Western blot was per-formed to observe the level of phospho -JNK and phos-pho-c-Jun in cultured astrocytes. Immunofluorescent technology was performed to observe the level of phos -pho-αctivating transcription factor-2 ( ATF-2 ) protein expression. Results LPS induced a significant increase in phospho-JNK and phospho-c-Jun protein expression without affecting total protein levels , and increased the expression of phospho-αTF-2 and the release of TNF-α and NO in cultured astrocytes in rats. SP600125 significantly inhibited LPS-induced increasein the release of TNF-α and NO. SAaB obviously inhibited LPS -induced increase in the release of TNF-α and NO and the expression of phospho-JNK, phospho-c-Jun and phospho-αTF-2 protein in cultured astrocytes. Conclusion This study indicates that SAaB can inhibit the release of TNF-α and NO induced by LPS in cultured astrocytes through the suppression of JNK sig -nal transduction pathway activity.关键词
知母皂苷/脂多糖/星形胶质细胞/炎症因子/c-Jun氨基末端激酶/转录因子c-Jun/活化转录因子-2Key words
SAaB/ LPS/ astrocyte/ inflammatory factors / JNK/ c-Jun/ ATF-2分类
医药卫生引用本文复制引用
刘卓,隋海娟,闫恩志,刘婉珠,金英..知母皂苷对脂多糖引起星形胶质细胞炎症因子释放的影响及机制[J].中国药理学通报,2012,28(7):970-974,5.基金项目
辽宁省教育厅创新团队项目(No LT2010064) （No LT2010064）
辽宁省教育厅一般项目(NoL2011143) （NoL2011143）
辽宁医学院院内资助课题(No Y2010Z003) （No Y2010Z003）
