中国药理学通报2012，Vol.28Issue(7)：1019-1023,5.DOI:10.3969/j.issn.1001-1978.2012.07.031
白香丹含药血清对大鼠海马神经元中5-羟色胺2C受体的表达及其下游信号通路中IP3的影响
Effect of Baixiangdan on the expression of 5-HTR2C and the concentration of IP3 in neonate rat hippocampal neurons cultured in vitro
摘要
Abstract
Aim To explore the intervention mechanisms of baixiangdan capsule by observing the changes of the expression level of 5-HTR2C and the concentration of IP3 in neonate rat hippocampal neurons culturedin vitro. Methods Premenstrual syndrome ( PMS ) model rats with liver-qi invasion were induced by multiple emotional stimulations and baixiangdan capsule was administered after PMS induction. The medicatedserum was used to neonate rat hippocampal neurons . The expression level of 5-HTR2C in the neurons was e- valuated by Western blot and the concentration of IP 3 was measured with the Elisa method. Results Compared with control group , both the protein expression of 5-HTR2C and the concentration of IP 3 of PMS liver-qi invation group were significantly higher in neurons (P < 0. 01, P < 0. 01 ). After treated with baixiangdan medicated serum, the expression of 5-HTR2C and the concentration of IP3 in hippocampal neurons were significantly improved(P<0. 01,P<0. 01). ConclusionsPMS model rats with liver-qi invasion serum can up-regulate the expression of 5-HTR2C in hippocampal neurons and increase the concentration of the second messenger IP3. Baixiangdan capsule might partially play its role in ameliorating PMS by regulating the expression of 5-HTR2C and changing the concentration of IP3.关键词
白香丹胶囊/经前期综合征肝气逆证/大鼠/海马神经元/含药血清/5-HTR2C/IP3Key words
baixiangdan capsule/ premenstrual syndrome ( PMS) model rats with liver-qi invasion/ rat/ hippocampal neurons / medicated serum /5-HTR2C/IP3分类
医药卫生引用本文复制引用
柳新,薛玲,高杰,魏盛,谭倩,苏云祥..白香丹含药血清对大鼠海马神经元中5-羟色胺2C受体的表达及其下游信号通路中IP3的影响[J].中国药理学通报,2012,28(7):1019-1023,5.基金项目
国家重点基础研究发展计划(973计划)资助课题(No 2011CB505102) （973计划）
国家自然科学基金重点项目(No 30930110) （No 30930110）
