中国医学科学院学报2012，Vol.34Issue(2)：126-129,4.DOI:10.3881/j.issn.1000-503X.2012.02.005
盐酸阿比朵尔体外抑制2009新型流感病毒A(H1N1)的作用
The in-vitro Effects of Arbidol Hydrochloride Against 2009 New Influenza Virus A (H1N1)
张兴权 1代军朋 2蔡柏蔷3
作者信息
- 1. 美国加州大学圣地亚哥医学院传染病系,拉霍亚,加州92093
- 2. 石家庄四药有限公司,石家庄,052165
- 3. 中国医学科学院 北京协和医学院 北京协和医院呼吸内科,北京,100730
- 折叠
摘要
Abstract
Objective To detect the in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1) . Methods The activity of arbidol hydrochloride against 2009 new influenza virus A (H1N1) was determined in MDCK cell cultures. Hemagglutination assay, observation of cytopathic effects, RT-PCR and quantitative RT-PCR tests were performed for determination of virus titers. Inhibition concentration 50% and cy-totoxic concentration 50% were calculated with Chou's Menu of Dose-Effect Program. Results Arbidol hydrochloride showed low cytotoxicity (cytotoxic concentration 50% > 100 μmol/L) and significant anti-2009 new influenza virus A (H1N1) activity in cell cultures. Inhibition concentration 50% were (5. 5 ±0. 9) , (3. 4 ±0. 8) , and (1. 5 ±0. 2) μmol/L in hemagglutination assay, cytopathic effect test, and quantitative RT-PCR assay, respectively. Conclusion Arbidol has low cytotoxicity and high anti-virus activity and can effectively trigger the activities of interferon and immune response, and therefore can be a valuable anti-influenza virus drug.关键词
盐酸阿比朵尔/2009新型流感病毒A(H1N1)/RT-PCRKey words
arbidol hydrochloride/2009 new influenza virus A (H1N1) /RT-PCR分类
医药卫生引用本文复制引用
张兴权,代军朋,蔡柏蔷..盐酸阿比朵尔体外抑制2009新型流感病毒A(H1N1)的作用[J].中国医学科学院学报,2012,34(2):126-129,4.
