中国医学装备2012，Vol.9Issue(3)：4-8,5.
不同剂量过敏原对滴鼻制备急性和亚急性小鼠哮喘模型的影响
Effects of different doses of allergen on acute and subacute mouse models of asthma
摘要
Abstract
Objective： To evaluate effects of different doses of allergen on ovalbumin （OVA）-induced acute and sub-acute mouse models of asthma by intranasal challenge. Methods： In brief, intranasal inhalation challenge with different doses of OVA （25, 50 and 100μg, respectively） was performed to induce acute and sub-acute mouse models of asthma. The respiratory function parameters of excited group and control group were measured using an invasive pulmonary function device. After collecting bronchoalveolar lavage fluid （BALF） of each group the cells were counted. Histological staining with HE, Congo red, Masson and Periodic acid-Schiff （PAS） were employed in paraffin-embedded sections derived from the left lungs to identify inflammatory cell infiltration, collagen deposition, mucus expression and other indicators. Results： Compared with 25 μg OVA- challenged group and saline group, at day of 20, 24, 100 and 50μg OVA-challenged groups showed the bronchial hyperresponsiveness and the elevated numbers of inflammatory cells including eosinophils in BAL fluid, the alveolar and bronchial wall, as well as in bronchial lumen and blood vessels. Bronchial mucosal folds were increased, extended and thickened, with a large amount of mucous sputum plugs in the bronchial lumens and significant deposition of collagen fibers in the bronchial and blood vessels. Additionally, PAS staining showed increased mucus in bronchial lumen. Conclusion： With different doses of intranasal inhalation of OVA, we successfully established varying degrees of acute and sub-acute mouse models of asthma. Our studies provided the foundation for further research on the pathogenesis and drug screening of asthma using mouse models.关键词
过敏原/哮喘模型/急性期/亚急性期Key words
Allergen/Asthma model/Acute phase/Sub-acute phase分类
医药卫生引用本文复制引用
姚秀娟,李艳,吕喆,袁慧慧,贾军会,赵文明,安云庆,王炜,黄克武,孙英..不同剂量过敏原对滴鼻制备急性和亚急性小鼠哮喘模型的影响[J].中国医学装备,2012,9(3):4-8,5.基金项目
国家自然科学基金 （）
北京市教育委员会科技发展计划面上项目基金 （）
北京市属高等学校人才强教深化计划“中青年骨干人才培养计划”项目基金 （）
首都医科大学科研基金 （）
首都医科大学基础临床合作资助基金 （）
