中国药业2012，Vol.21Issue(8)：20-23,4.
高效液相色谱-质谱联用法同时测定盐酸金刚烷胺和马来酸氯苯那敏的血药浓度
Simultaneous Determination of Amantadine Hydrochloride and Chlorpheniramine Maleate in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrum
聂朝霞 1李健和 2彭词艳 2易利丹 2曾小慧2
作者信息
- 1. 湖南省衡阳市妇幼保健院药剂科,湖南衡阳,421001
- 2. 中南大学湘雅二医院药学部,湖南长沙,410011
- 折叠
摘要
Abstract
Objective To establish a sensitive and rapid high performance liquid chromatographic - tandem mass spectrometric (HPLC -MS/MS) method for the simultaneous determination of amantadine hydrochloride and chlorpheniramine maleate in human plasma. Methods The C18 column was used with water (containing 0.5‰ formic acid): acetonitrile: methanol(18: 32: 50) as the mobile phase and finasteride as the internal standard. The concentration of amantadine hydrochloride and chlorpheniramine maleate in human plasma was determined by HPLC - MS / MS with selected reaction monitoring (SRM) mode via electrospray ionization (ESI) . Results The concentration of amantadine hydrochloride and chlorpheniramine maleate were linear in the range of 3.9-375.0 ng/mL and 0.2-19.2 ng/mL,respectively. The relative recovery rates of amantadine hydrochloride were 106.4% (low), 94. 9% (middle) and 93.1% (high), respectively and its intra and inter - day RSD were 2. 6% ,3. 2% ,4. 1% and 4. 3% ,5. 4% ,5. 6% respectively. Its lowest limit of quantification was 3.9 ng/mL with RSD 3.1%. The relative recovery rates of chlorpheniramine maleate were 98. 9% (low), 97.9% (middle) and 101.8% (high) respectively and its intra and inter-day RSD were 8. 5% , 8. 0% ,4. 1% and 6. 9%, 5. 5%, 5.6% ,respectively. Its lowest limit of quantification was 0.2 ng/mL with RSD 1.7%. Conclusion The established method is highly sensitive, simple and accurate, which can be suitable in simultaneous quantification of amantadine hydrochloride and chlorpheniramine maleate in human plasma and may be used for the study of pharmacokinetics of amantadine hydrochloride and chlorpheniramine maleate.关键词
高效液湘色谱-质谱联用法/盐酸金刚烷胺/马来酸氯苯那敏/血浆药物浓度Key words
HPLC-MS/MS/ amantadine hydrochloride/ chlorpheniramine maleate/ plasma drug concentration分类
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聂朝霞,李健和,彭词艳,易利丹,曾小慧..高效液相色谱-质谱联用法同时测定盐酸金刚烷胺和马来酸氯苯那敏的血药浓度[J].中国药业,2012,21(8):20-23,4.
